Agilent Seahorse Wave Error exporting to GraphPad Prism: Troubleshooting Tips

Document created by courtneynadeau Employee on Oct 15, 2019Last modified by courtneynadeau Employee on Oct 18, 2019
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If the Wave software used with Seahorse data displays an error stating: “The file cannot be opened since it is damaged or truncated”, this document can be used to help troubleshoot.


Here is a list of the most common causes for this error:

  • No background correction groups assigned. For any Seahorse assay, background wells are critical for acquiring reliable data.  Background correction wells are assigned to the plate map by default.  Although it is possible to modify the default background wells, at least one well must be assigned to the background correction group


Try these steps for a possible resolution:

  • If assigning at least one background correction well does not remedy the issue, contact Agilent Cell Analysis technical support at:
    • Email contact:
    • Phone Contact:
      • US Contact: 1 800 227 9770 option 3, 8
      • UK Contact: 0 800 096 7632 option 3
      • DE Contact: 0 800 180 66 78 option 3
      • NL Contact: 0 800 022 7243 option 3
      • Other European Countries Contact: 45 3136 9878 option 3


More Troubleshooting Tips: Cell Analysis FAQs and Troubleshooting


Keywords: Prism error, Prism, GraphPad, GraphPad Error, Export Error, Seahorse, XFe96, XFe24, XFp, XF24, XF96, Troubleshooting