What do I do if I cannot detect any signal in wells containing cells and pH-Xtra (or I can detect a signal but the slope (rate) appears very low)?
Check correct Instrument Settings (“Appendix A - Instrument Settings” on page 29)- Perform Signal Optimization - Include Signal Controls as described previously - Increase cell density. Check pH of prewarmed Respiration Buffer and correct as necessary, as pH can drift over time. If tested and not resolved, contact www.agilent.com.
What do I do if I can detect a signal in wells containing cells and pH-Xtra, but the slope (rate) falls initially or is variable from well to well?
Check cell seeding and pipetting consistency; increase cell density; ensure plate, instrument, and all culture media and stock solutions are prewarmed at 37 °C before use; reduce plate preparation times; reduce assay volume to 60 μL.
More FAQs: Cell Analysis FAQs and Troubleshooting
Keywords: pH-Xtra, pH Xtra Glycolysis Assay, Signal, Slope, Signal Optimization, Soluble Sensors, FAQ