Agilent Seahorse XF Real-Time ATP Rate Assay Report Generator FAQs

Document created by courtneynadeau Employee on Oct 15, 2019Last modified by courtneynadeau Employee on Oct 16, 2019
Version 3Show Document
  • View in full screen mode

What rate measurements are used to calculate the parameters in this Report Generator?

 Parameter equations are described in Table 1 on page 6 and Table 2 on page 17 of the Report Generator User Guide.


How do I remove outlier wells in the Report Generator?

 Outlier assay wells must be turned OFF or reassigned in Wave 2.6 before Excel export. See “Exclude Outliers/Groups from Analysis” on page 19, or the Wave User Guide for more information.


Can I use the Excel file exported from the XFp or XF96 Analyzer?

Excel files exported from earlier versions of Wave (Desktop or Controller) and XF96/XF24 software are not compatible. If the Excel file has been exported from Wave but cannot be imported to the Report Generator, please contact Agilent Seahorse Technical Support.


If you receive an error message about Instrument Protocol (XFe96; XFe24; XF96 only):

Custom Cycles are not part of the standardized assay template for the XF Real-Time ATP Rate Assay and not supported in Report Generator analysis. A Custom Cycle refers to an extra 'Mix' or 'Wait' command step in the Instrument Protocol an assay. Please contact Agilent Seahorse Technical Support if you have any additional questions regarding Custom Cycles.


Can I use baseline rate data (%) to calculate assay parameters?

Normalized (or nonnormalized) ECAR data is used for parameter calculations. Parameter calculations using baseline rate data (%) is not currently supported.


Why is there no glycoATP Production Rate data?

The XF Real-Time ATP Rate Assay Report Generator uses PER data exported from Wave 2.6 to calculate glycoATP Production Rates. If Buffer Factor is not assigned to assay media in Wave 2.6 before export, then the Report Generator will not calculate glycoATP Production Rates. Open your assay result file in Wave 2.6 and assign buffer factor to the assay media used and then re-export data to Excel. For instructions on assigning buffer factor to assay media in Wave 2.6 software, see the Quick Reference Guide: Calculating PER Data.


Can I analyze multiple result files in this Report Generator?

No, the XF Real-Time ATP Rate Assay Report Generator enables the import and analysis of individual assay result file. Analysis of multiple result files must be done manually.


More FAQs: Cell Analysis FAQs and Troubleshooting


Keywords: ATP Rate Assay, ATP Rate Assay Report Generator, glycoATP, Outlier, Parameter Equations, Report Generator, Report Generator Error, Seahorse, XFe96, XFe24, XFp, FAQ