Agilent Seahorse Mito Stress Test Single-File and Mulit-File XF Report Generator FAQs

Document created by courtneynadeau Employee on Oct 15, 2019Last modified by courtneynadeau Employee on Oct 17, 2019
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What rate measurements are used to calculate the parameters in this Report Generator?

Parameter equations are described in Table 1 of the user guide.


How do I remove outlier wells in the Report Generator?

Outlier assay wells must be turned OFF or reassigned in Wave 2.3 before Excel export. See section called Exclude Outliers/Groups from Analysis or the Wave User Guide for more information.


Can I use the Excel file exported from the XFp, XF96, or XF24 Analyzer?

It is recommended to export assay result data directly to the XF Report Generator from Wave 2.3 (Desktop or Controller), however data can also be manually imported as an Excel file (*.xlsx). Excel files exported from earlier versions of Wave (Desktop or Controller) and XF96/XF24 software are not compatible. If the Excel file has been exported from Wave but cannot be imported to the Report Generator, please contact Seahorse Technical Support.


If you receive an error message about Instrument Protocol (XFe96; XFe24; XF96; XF24 only)

Errors upon data import into the Report Generator are likely caused by a custom cycle in the Instrument Protocol. A Custom Cycle refers to an extra ‘Mix’ or ‘Wait’ command step in the Instrument Protocol an assay. Custom Cycles are not part of the standardized assay template for the Seahorse XF Cell Mito Stress Test and not supported in Report Generator analysis. Please contact Seahorse Technical Support if you have any additional questions regarding Custom Cycles.


Can I use baseline rate data (%) to calculate assay parameters?

Normalized (or nonnormalized) OCR data is used for parameter calculations, parameter calculations using baseline rate data (%) is not supported currently.


Where is my ECAR data?

ECAR (Extracellular Acidification Rate) data is displayed on the Measures Sheet tab in the Seahorse XF Cell Mito Stress Test Report Generator.


How many Excel files can be imported for analysis? (MULTI-FILE ONLY)

  • XF/XFe96 & XF/XFe24 – A maximum of five Excel files (*.xlsx) can be imported to the Multi-File XF Report Generator.
  • XFp – A maximum of 10 Excel files (*.xlsx) can be imported to the Multi-File XF Report Generator.


Can I import Excel files generated on two different XF analyzers? (MULTI-FILE ONLY)

No. Assay result data must be generated by the same Agilent Seahorse XF, XFe, or XFp Analyzer – combining data from different analyzers is not supported currently (example: XF96 and XFe96).


More FAQs: Cell Analysis FAQs and Troubleshooting


Keywords: Mito Stress Test, Report Generator, Parameter Equations, Report Generator Error, Seahorse, XFe96, XFe24, XFp, XF24, XF96, FAQ