Agilent Seahorse XF Cell Energy Phenotype Test Report Generator FAQs

Document created by courtneynadeau Employee on Oct 15, 2019Last modified by courtneynadeau Employee on Oct 16, 2019
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What rate measurements are used to calculate the parameters in this Report Generator?

Parameter equations are described in Table 1 on page 6 of the user guide.


How do I remove outlier wells in the Report Generator?

Outlier assay wells must be turned OFF, or reassigned in Wave 2.3 before Excel export. See the “Exclude Outliers/Groups from Analysis ” on page 19 or the Wave User Guide for more information.


Can I use the Excel file exported from the XFp, XF96, or XF24 Analyzer?

It is recommended to export assay result data directly to the XF Report Generator from Wave 2.3 (Desktop or Controller), however data can also be manually imported as an Excel file (*.xlsx). Excel files exported from earlier versions of Wave (Desktop or Controller) and XF96/XF24 software are not compatible. If the Excel file has been exported from Wave but cannot be imported to the Report Generator, please contact Agilent Seahorse Technical Support.


If you receive an error message about Instrument Protocol (XFe96; XFe24; XF96; XF24 only). Errors upon data import into the Report Generator are likely caused by a custom cycle in the Instrument Protocol. A Custom Cycle refers to an axtral Mix or Wait command step in the Instrument Protocol an assay. Custom Cycles are not part of the standardized assay template for the Agilent Seahorse XF Cell Energy Phenotype Test and not supported in Report Generator analysis. Please contact Agilent Seahorse Technical Support if you have any additional questions regarding Custom Cycles.


Can I use baseline rate data (%) to calculate assay parameters?

Normalized (or nonnormalized) OCR data are used for parameter calculations; parameter calculations using baseline rate data (%) are not supported currently.


Where can I find the OCR and ECAR kinetic graphs?

Both OCR and ECAR kinetic graph data are displayed on the Measures Sheet tab in the Agilent Seahorse XF Cell Energy Phenotype Report Generator.


How do I analyze multiple result files in a Report Generator?

The Agilent Seahorse Multi-File XF Cell Energy Phenotype Report Generator enables the import and analysis of multiple XF Cell Energy Phenotype Test (and XF Cell Mito Stress) result files. Find out more and download the Multi-File XF Report Generator.


More FAQs: Cell Analysis FAQs and Troubleshooting


Keywords: Cell Energy Phenotype, Phenotype, Report Generator, Outlier, Report Generator Error, Seahorse, XFe96, XFe24, XFp, XF24, XF96, FAQ