Collection of Cell Analysis Resources

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Here is a "best of" collection of links to manuals, tools, and other resources for Agilent Cell Analysis products. For other instruments refer to Collection of Support Resources.

 

 

Collection of Cell Analysis Resources

 

 

Seahorse

 

Overall:

 

Cell Analysis FAQs and Troubleshooting

Agilent Seahorse XF Media Selection Guide

Seahorse XF Assay Learning Center

Preparation of XF Assay Media

Seahorse XF Assay Guides and Templates

Agilent Cell Reference Database

Publications with Agilent Cell Analysis Data

Knowing your Cells of Interest: Advice and Suggestions for a Successful XF Experience

Agilent Seahorse Software Specifications

 

XFp

Instrument User Guide

XFp Extracellular Flux Analyzer User Guide

 

XFp Kits – User Guides

Agilent Seahorse XFp Real-Time ATP Rate Assay Kit

Agilent Seahorse XFp Cell Mito Stress Test Kit

Agilent Seahorse XFp Glycolytic Rate Assay Kit

Agilent Seahorse XFp Cell Energy Phenotype Test Kit

Agilent Seahorse XFp Mito Fuel Flex Test Kit

Agilent Seahorse XF Palmitate-BSA FAO Substrate Guide

Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) Guide

Agilent Seahorse XFp Glycolysis Stress Test Kit

 

Site Preparation Checklist

XFp Site Preparation Checklist

 

 

Overall User Guides

Cell Characterization: The XFp Analyzer and the XF Real-Time ATP Rate Assay

Seeding Adherent Cells in Agilent Seahorse XFp Cell Culture Miniplates

How to Hydrate an Agilent Seahorse XFp Sensor Cartridge

Washing Adherent Cells in Agilent Seahorse XFp Cell Culture Miniplates

Seeding Suspension Cells in Agilent Seahorse XFp Cell Culture Miniplates

Loading the Agilent Seahorse XFp Sensor Cartridge Injection Ports

 


XF/XFe

XFe Instrument User Guide

XFe Extracellular Flux Analyzer User Guide (Version 2.6)

 

XFe Kits – User Guides

Agilent Seahorse XF Real-Time ATP Rate Assay Kit

Agilent Seahorse XF Palmitate-BSA FAO Substrate Quickstart Guide

Agilent Seahorse XF Cell Mito Stress Test Kit

Agilent Seahorse XF Glycolytic Rate Assay Kit

Agilent Seahorse XF Cell Energy Phenotype Test Kit

Agilent Seahorse XF Mito Fuel Flex Test Kit

Agilent Seahorse XF Palmitate-BSA FAO Substrate Guide

Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) Guide

Agilent Seahorse XF Glycolysis Stress Test Kit

 

Site Preparation Checklists

XFe96 Site Preparation Checklist

XFe24 Site Preparation Checklist

 

 

Normalization – User Guides

Seahorse XF Imaging and Normalization System XFe24 Hoechst33342 Staining Optimization Guidelines

Seahorse XF Imaging and Normalization System XFe96 Hoechst33342 Staining Optimization Guidelines

 

Hydration – User Guides

How to Hydrate an Agilent Seahorse XFe96 Sensor Cartridge

How to Use the Hydro Booster for Improved Hydration and Data Quality on the Agilent Seahorse XFe 24 Analyzer

 

Loading Injection Ports – User Guides

Loading the Agilent Seahorse XF24 Sensor Cartridge Injection Ports

Loading the Agilent Seahorse XFe96 Sensor Cartridge Injection Ports

Loading the Agilent Seahorse XFe24 Sensor Cartridge Injection Ports

 

Informative and How-To Videos

Bravo Automated Workflow for Seahorse XFe96 Sample Preparation

How to analyze Agilent XF Real-Time ATP Rate Assay result data

Streamline your cell analysis

Energy Pathways Video

Agilent Seahorse XF Analyzer Multi-port Drug Injection Video

Measuring Cell Metabolism - Transient Micro-chamber Video

 

Wave

Agilent Seahorse XFe96 and XFe24 Analyzers (For use with Wave Desktop 2.4) User Guide

Wave Desktop 2.4 ReadMe

Wave 2.4 Release Notes

Wave 2.6 User Guide For use with Wave Desktop software and Agilent Seahorse XFe Analyzers

Wave Desktop 2.6 Read Me

Wave 2.6 Release Notes

Running Wave Desktop 2.6 on a Mac

Download Hypoxia License Installer

 

 

Articles and Whitepapers:

Improving Quantification of Cellular Glycolytic Rate Using Agilent Seahorse XF Technology (Link to download, not straight to paper)

Quantifying Cellular ATP Production Rate Using Agilent Seahorse XF Technology

 

Technical Overviews

Immobilization of Non-Adherent Cells with Cell-Tak for Assay on the Agilent Seahorse XFe/XF96 or XFp Analyzer Application Notes

Immobilization of Non-Adherent Cells with Cell-Tak for Assay on the Agilent Seahorse XFe/XF24 Analyzer

Modifying Assay Guides for use with Agilent Seahorse XFe24 and XF24 Analyzers

Assessing Mitochondrial Respiratory Complexes Using Cells Permeabilized with XF Plasma Membrane Permeabilizer (PMP)

Islet Assay Using the Agilent Seahorse XF24 Islet Capture Microplate

Data Quality Management using Brightfield Images with the Seahorse XF Imaging and Normalization System

Using PMP to Measure Mitochondrial ETC Complex Activity in Limited Biomaterials

3T3-L1 Pre-Adipocyte Differentiation

Methods and strategies for normalizing XF metabolic data to cellular parameters

Conducting an XFe Assay in an Hypoxia Chamber at ≥3% O² *

Using PMP to Measure Substrate Specific ETC/OxPhos Activity in Permeabilized Cells

 

Application Notes

Application Notes (overall link)

Assessing functional metabolism using cells sourced from blood

Assessing mitochondrial dysfunction in primary cardiomyocytes

Assessing the impact of autophagy on cellular metabolism

Assessing therapies for traumatic brain or spinal cord injuries

Bioenergetic analysis of primary neuronal deficiencies

Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes

Cancer Cells Evoke Flexibility By Maintaining a Bioenergetic Balance Between Glycolysis and Mitochondrial Respiration Linked to Metastatic Potential

Characterization of Glycolysis with a Panel of Common Cellular Models Using Agilent Seahorse XF Technology

Defining metabolic phenotypes in cancer cells

Determining Metabolic Switch in Induced Pluripotent Stem Cells (iPSCs)

Direct Measurements of Cellular Metabolism for Identification of Mitochondrial Drug Targets

Evaluating changes in cell metabolism in neuroimmune and neuropsychiatric disorders

Functional validation of Genomic and Proteomic data

Identifying Metabolic Phenotype Switches in Cancer Cells Using the Agilent Seahorse XF Analyzer in an Hypoxic Environment

Measuring bioenergetic profiles of human adipocytes

Measuring functional metabolism of brite adipocytes

Measuring glycolysis and oxidative metabolism in cancer cells

Measuring glycolytic function in cells

Measuring Mitochondrial Respiration in Intact Skeletal Muscle Fibers with an Agilent Seahorse XF24/XFe24 Analyzer

Measuring the metabolic switch in cancer cells

Metabolic Pathway Determination Using the Seahorse XF24 Analyzer

Normalization of Agilent Seahorse XF Data by In-situ Cell Counting Using a BioTek Cytation 5

Quantitative Analysis of Neutrophil Activation Using Agilent Seahorse XF Technology

Rapid, Real-time Detection of T Cell Activation Using an Agilent Seahorse XF Analyzer

Real Time Discrimination of Inflammatory Macrophage Activation Using Agilent Seahorse XF Technology

Simultaneously Measuring Oxidation of Exogenous and Endogenous Fatty Acids Using the XF Palmitate-BSA FAO Substrate with the Agilent Seahorse XF Cell Mito Stress Test

XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System

 

Quick Reference Guides

Quick Reference Guide: Calculating Proton Efflux Rate (PER) Data

Agilent Seahorse XF Buffer Factor Protocol Quick Reference Guide

 

Webinars

Webinars (overall link)

Abnormal Respiratory Function in Autism Immune Cells

Adaptation of Microplate-based Respirometry for Hippocampal Brain Slices

An MTCH2 pathway repressing mitochondria metabolism regulates haematopoietic stem cell fate

Analysis of Glutaminolysis and Fumarate Respiration in Bcl-xL-expressing Cells

Assessing bioenergetic function in response to oxidative stress by metabolic profiling

Assessment of metabolic reprogramming of the skin fibroblasts from patients with mitochondrial diseases using the Seahorse XF Analyzer

Assaying Isolated Mitochondria in the Seahorse XF24: Learning More while Requiring Less

Beyond Lactate: Measuring Glycolytic Rates in Live Cells with the Agilent Seahorse XF Glycolytic Rate Assay

Biochemical analysis in C. elegans as a model for metabolic disease and aging

Bioenergetic Evaluation in Mitochondrial Disorders

Characterization of Potential Bioenergetic Medicine Agents

Combining Seahorse XF Analysis with Stable Isotope Tracing to Reveal Novel Metabolic Drug Targets

Defining the Critical Bioenergetic Function of Huntingtin Protein

De-risk Your Drug Discovery Pipeline

Determine the Metabolic Phenotype of Your Cells Using the XFp Cell Energy Phenotype Test Kit

Dissecting the role of energy metabolism and mitochondrial ROS in the familial autoinflammatory disease TRAPS

Drug Discovery: Natural Products that Expand and Induce Brown Adipose Tissue using innovative Stem Cell Metabolism Assays

Exploring how Mycobacterium tuberculosis reprograms host bioenergetic metabolism

Exploring the metabolism of CAR-T cells

Fueling T cells in Immunity and Cancer

How to Measure Mitochondrial Function in Whole Islets. Sensing the nutrient sensor: measuring bioenergetic parameters from intact mouse and human islets of langerhans

Human Disorders of Bioenergetics

Impaired mitochondrial ß-oxidation in cardiomyocytes: role of the cardiac renin-angiotensin system and miR-208

Importance of the Mitochondrial Reserve Capacity in Cardiovascular Cells Exposed to Oxidative Stress.

iPSC-derived neural cells for drug discovery of mitochondrial disorders

It is time for a new measure of cell function: Quantifying Cellular ATP Production Rate Using Agilent Seahorse XF Technology

Measuring ATP production rates with the Seahorse XF Analyzer

Measuring cells' dependency and flexibility on the three most abundant fuels in the body: glucose, glutamine and fatty acids

Measuring Functional Metabolism of Tissues in 3D

Measuring platelet bioenergetic function - from marker to mechanism of disease

Metabolic Adaptations in Fumarate Hydratase Deficient Cells

Metabolic Plasticity as a Therapeutic Target

Metabolic plasticity in T cell responses: Implications for adaptive immunity

Metabolic reprogramming as a therapeutic strategy for cutaneous squamous cell carcinoma

Metabolic reprogramming in macrophages during inflammation

Metabolic Reprogramming Therapy in the Treatment of Cancer

Metabolic switches that drive stem cell specification and differentiation to a mature neuron

Metabolomics - Following the Carbons that Fuel Cancer

Mitochondria Regulate Cancer

Mitochondria - the Powerhouse of the Cell Goes from Bench to Bedside

Mitochondrial Bioenergetics in Diabetic Cardiomyopathy

Mitochondrial Control in Health & Disease

Mitochondrial pyruvate metabolism controls neuronal function and excitotoxic death

Mitochondrial Profiling in Cells Under Physiological Conditions and In Response to Stress

Multidimensional control of mitochondrial energy production: learning from physiology and pathology

New solutions for measuring metabolism; harnessing the power of your plate reader

Normalizing Your Seahorse XF Data with Automated Cell Counting

Probe the Metabolic Depths of Tumor Spheroids Using the XFe96 Analyzer

Profiling Mitochondrial Function has never been so Easy: A Tutorial on the New XF Mito Stress Test Kit

Programming the Mitochondrial Choice of Fuel Substrates: Metabolic Control of Neuronal Excitability

Rapid adoption of live cell kinetics and real time biology in the design and validation of adoptive T cell strategies

Regulation of cancer cell growth by metabolic substrates

Regulating oxygen metabolism can radiosensitize solid tumors

Quantifying macrophage activation using Agilent Seahorse XF technology

Quantitative analysis of neutrophil activation using Agilent Seahorse XF technology

Seahorse Metabolic Analyzers Enable Rapid, Quantitative Measurement of Cellular Bioenergetics

Simultaneously Measuring Oxidation of Exogenous and Endogenous Fatty Acids

Systems-based approach to characterizing human pluripotent stem cell metabolism

T cell metabolism in activation and exhaustion

The Hitchhiker's Guide to XF Bioenergetic Assays

The Mitochondrial Connection from Bench to Bedside: Strategies to Prevent, Delay and Treat Alzheimer's Disease

The Rate and Coupling Efficiency of Oxidative Phosphorylation in Mitochondria and Cells

Type 1 Interferons Induce Changes in Core Metabolism that Are Critical for Immune Function

Tutorial: Measuring Bioenergetics in a Variety of Cell Types

Tutorial 2: Measuring Bioenergetics in Primary Cell Types

Understanding the tumor microenvironment, and its effect on tumor metabolism, progression and therapy

Use of Agilent XF Seahorse technology to monitor metabolic reprogramming via OXPHOS blockade in leukemia

Using a novel permeabilization reagent to uncover mechanisms of mitochondrial dysfunction

Using Reserve Capacity to Screen for Mitochondrial Toxicities and Biogenesis

Using the XF Analyzer to measure cellular respiration and glycolysis under hypoxic conditions

What's Fueling Mitochondria? Designing and Interpreting Pathway-Specific XF Assays

Working Towards Defining a Metabolic Roadmap for Stem Cell Lineage Progression

XF Assay Tutorial: A rapid, real-time assay to monitor T cell activation using the Agilent Seahorse XFp Analyzer

XF Glycolysis Stress Test Assesses Glycolysis and Glycolytic Capacity in Cells

 

 

Report Generators – Single and Multi-file (if available)

Seahorse XF Cell Energy Phenotype Test Report Generators

Seahorse XF Cell Mito Stress Test Report Generators

Seahorse XF Glycolytic Rate Assay Report Generators

Seahorse XF Mito Fuel Flex Test Report Generator

Seahorse XF Real-Time ATP Rate Assay Report Generators

 

 

 

 

Soluble Sensors

 

Overall:

Plate Reader Compatibility

MitoXpress Xtra Oxygen Consumption Assay Plate Reader compatibility chart

Ph-Xtra Glycolysis Assay Plate Reader compatibility chart

MitoXpress Intra Plate Reader Compatibility Guide

 

Instrument Setup Guides – MitoXpress Xtra Oxygen Consumption Assay Setup Guides

MitoXpress Xtra Oxygen Consumption Assay Instrument setup guide for Perkin Elmer plate readers

MitoXpress Xtra Oxygen Consumption Assay Instrument setup guide for Tecan plate readers

MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for BMG Plate Readers

MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for Molecular Devices Plate Readers

MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for BioTek Plate Readers

 

Instrument Setup Guides – pH-Xtra Glycolysis Assay Setup Guides

pH-Xtra Glycolysis Assay Instrument setup guide for Perkin Elmer plate readers

pH-Xtra Glycolysis Assay Instrument setup guide for Tecan plate readers

pH-Xtra Glycolysis Assay Instrument Set Up Guide for BioTek Plate Readers

pH-Xtra Glycolysis Assay Instrument Set Up Guide for Molecular Devices Plate Readers

pH-Xtra Glycolysis Assay Instrument Set Up Guide for BMG Plate Readers

 

 

User Manuals

Agilent pH-Xtra Glycolysis Assay

Agilent MitoXpress Xtra Oxygen Consumption Assay (HS Method)

Agilent MitoXpress Intra Intracellular Oxygen Assay

 

Application Notes

Assessing Microbial Proliferation and Antibiotic Resistance using the MitoXpress Xtra Oxygen Consumption Assay

In vitro ischemia-reperfusion model with real-time monitoring of cellular oxygenation and reactive oxygen species generation

Screening for Drug-Induced Mitochondrial Dysfunction Using MitoXpress Xtra on Isolated Mitochondria

 

Software

Data Visualization Tool

Agilent MitoXpress & pH-Xtra Data Visualization Tool User Manual

 

Webinars

New solutions for measuring metabolism; harnessing the power of your plate reader

 

 

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