We developed a highly sensitive and specific LC/MS/MS method to quantitate norepinephrine, epinephrine, and dopamine in plasma. An Agilent Captiva NDLipids filtration and a selective solid phase extraction procedure was used to clean up plasma. The method achieved the required functional sensitivity and quantitated analytes over a sufficiently wide dynamic range. Reproducibility was excellent for all compounds (CV < 5 %). All calibration curves displayed excellent linearity, with R2 > 0.9997.
Publication number: 5991-6530EN
Publication Date: January 15, 2016