Plasma Catecholamines by LC/MS/MS Using Agilent Captiva NDLipids, BondElut PBA SPE, 1290 Infi nity LC, and 6460 Triple Quadrupole LC/MS

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    Application Note

    Clinical Research

     

    Abstract

    We developed a highly sensitive and specific LC/MS/MS method to quantitate norepinephrine, epinephrine, and dopamine in plasma. An Agilent Captiva NDLipids filtration and a selective solid phase extraction procedure was used to clean up plasma. The method achieved the required functional sensitivity and quantitated analytes over a sufficiently wide dynamic range. Reproducibility was excellent for all compounds (CV < 5 %). All calibration curves displayed excellent linearity, with R2 > 0.9997.

     

    Publication number: 5991-6530EN

    Publication Date: January 15, 2016

     

    http://www.agilent.com/cs/library/applications/5991-6530EN.pdf