Plasma Metanephrines and 3-Methoxytyramine by LC/MS/MS Using Agilent SampliQ WCX SPE, 1290 Infinity LC, and 6460 Triple Quadrupole LC/MS

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    Application Note

    Clinical Research



    We developed a highly sensitive and specific LC/MS/MS method to quantitate metanephrine, normetanephrine, and 3-methoxytyramine in plasma. A solid phase extraction (SPE) procedure was used to extract the analytes and remove plasma interferences. The method achieved the required functional sensitivity, and quantitated analytes over a sufficiently wide dynamic range. Reproducibility was excellent for all compounds (CV < 6 %). All calibration curves displayed excellent linearity, with R2 > 0.9996.


    Publication number: 5991-6531EN

    Publication Date: February 25, 2016