Biotherapeutics & Biologics
Hydrophobic interaction chromatography (HIC) is a popular approach for both downstream processing and analytical scale analysis of monoclonal antibodies (mAbs). In the latter case, HIC is a useful characterization tool because it can often separate mAb variants, for example certain isomers or oxidized species. HIC separations are also gaining popularity for the characterization of antibody-drug conjugates (ADCs). Such investigations often benefit from mass measurement using MS, but the high salt conditions used for HIC separations are completely incompatible with ESI. In this work, we overcome this obstacle using a 2D-LC system, which affords multiple heart-cutting and subsequent desalting/separation using reversed-phase chromatography on-line with TOF MS.
Publication number: 5991-6376EN
Publication Date: January 26, 2016