Determination of Sweeteners, Preservatives, and Caffeine in Various Food and Consumer Products Using the Agilent 1290 Infinity II LC

Version 1

    Application Note

    Food Testing and Agriculture



    The Agilent 1290 Infi nity II LC was used to analyze additives such as sweeteners, preservatives, and caffeine in various food products, beverages, and consumer toothpaste. The developed method facilitates accurate and sensitive determination of nine additives using a diode array detector (DAD) and an evaporative light scattering detector (ELSD) placed in series. After the DAD, a valve was installed to remove nonretained solutes in reversed-phase LC, which could increase the noise level of the ELSD. Total analysis time, including re-equilibration, was below 10 minutes with minimal sample preparation. Method performance was evaluated with standard solutions as well as with a series of real samples.


    Publication number: 5991-6580EN

    Publication Date: February 1, 2016