Agilent 1290 Infinity II LC with ISET – Emulation of the Agilent 1100 Series LC Through Waters Empower Software Analysis of an Analgesic Mixture

Version 1

    Application Note

    Small Molecule Pharmaceuticals



    The Agilent Intelligent System Emulation Technology (ISET) enables seamless transfer of methods from HPLC systems such as the Agilent 1100 Series Binary LC to the Agilent 1290 Infinity II LC. With ISET, the same retention times and peak resolution can be obtained without any method or hardware changes. This Application Note demonstrates the use of ISET through Waters Empower software with Agilent Instrument Control Framework for the analysis of five analgesics. The mixture was analyzed using an 1100 Series Binary LC and a 1290 Infinity II LC with ISET enabled. On both systems, the achieved retention times and resolution values showed excellent agreement.


    Publication number: 5991-6541EN

    Publication Date: March 1, 2016