Fast Determination of the Bitter and Clove-like Flavor in Beer with the Agilent 1290 Infinity II LC

Version 1

    Application Note

    Food Testing and Agriculture

     

    Abstract

    The Agilent 1290 Infinity II LC was used to determine the bitter compounds iso-alpha-acids and reduced iso-alpha-acids, and the clove-like phenolic flavor 4-vinylguaiacol in bottled beer. A six-minute UHPLC method was developed to measure these important solutes in a single run. Diode array and fluorescence detectors (DAD and FLD) were placed in-series for quantification of iso-alpha-acids and reduced iso-alpha-acids, and of 4-vinylguaiacol, respectively. The fluorescence detector provided a selective, sensitive, and accurate determination of ppb amounts of 4-vinylguaiacol. Some figures of merit are presented together with the profiles of a series of different beers.

     

    Publication number: 5991-6665EN

    Publication Date: March 1, 2016

     

    http://www.agilent.com/cs/library/applications/5991-6665EN.pdf