Food Testing and Agriculture
The Agilent 1290 Infinity II LC was used to determine the bitter compounds iso-alpha-acids and reduced iso-alpha-acids, and the clove-like phenolic flavor 4-vinylguaiacol in bottled beer. A six-minute UHPLC method was developed to measure these important solutes in a single run. Diode array and fluorescence detectors (DAD and FLD) were placed in-series for quantification of iso-alpha-acids and reduced iso-alpha-acids, and of 4-vinylguaiacol, respectively. The fluorescence detector provided a selective, sensitive, and accurate determination of ppb amounts of 4-vinylguaiacol. Some figures of merit are presented together with the profiles of a series of different beers.
Publication number: 5991-6665EN
Publication Date: March 1, 2016