Agilent Intelligent System Emulation Technology (ISET) within the Agilent 1290 Infinity II LC facilitates the transfer of methods from Agilent and non-Agilent HPLC and UHPLC systems. This Application Note shows how the 1290 Infinity II LC with ISET emulates the ternary gradient of a Waters Acquity H-Class system for the analysis of peptides. Over 97 % agreement is achieved using the 1290 Infinity II LC with ISET, in contrast to without ISET.
Publication number: 5991-6715EN
Publication Date: April 1, 2016