Aflatoxin Analysis in Infant Formula with Enhanced Matrix Removal— Lipid by LC/MS/MS

Version 2

    Application Note

    Food Testing



    Aflatoxin M1 is the primary aflatoxin found in milk. It has European Commission (EC) maximum recommended levels of as low as 0.025 µg/kg in infant formula in Europe. In the United States, Food and Drug Administration (FDA) action levels for the aflatoxin are as low as 0.5 µg/kg in milk. This application note describes the determination of aflatoxins M1, G2, G1, B2, and B1 in infant formula by LC/MS/MS using Agilent Bond Elut Enhanced Matrix Removal—Lipid (EMR—Lipid). This study employed a QuEChERS extraction, followed by cleanup with EMR—Lipid dispersive SPE (dSPE). This method delivers excellent recoveries (88–113%), and precision (RSDs = 1.3–13.6%) for all aflatoxins at all levels. Due to the extensive matrix removal, limits of quantitation (LOQs) for this method were extended to below regulatory limits for both the U.S. and Europe. This simple and robust method requires minimal equipment and expertise, allowing for easy implementation in food laboratories.


    Publication number: 5991-6818EN

    Publication Date: May 1, 2016