Removal of Lipids for the Analysis of Toxicological Compounds in Plasma by LC/MS/MS -- Enhanced Matrix Removal-Lipid Dispersive Cleanup

Version 1

    Application Note

    Small Molecule, Toxicology



    A convenient analytical method for the determination of toxicological compounds in plasma involves the addition of acetonitrile to a small volume of plasma. The mixture is vortexed to elicit protein precipitation, and centrifuged. The supernatant is transferred to a dispersive tube containing Enhanced Matrix Removal—Lipid (EMR—Lipid) sorbent to remove >97% endogenous plasma lipid matrix components. EMR—Lipid removed substantially more lipids than other phospholipid removing sorbents tested. Analytes are isolated from spiked plasma samples with accuracies above 95% and Relative Standard Deviations (RSDs) of <6% on average. Combining protein precipitation with EMR—Lipid dispersive SPE in plasma offered separation for various toxicological compounds, Limits of Quantification (LOQs) at 1 ng/mL or below based on method performance. The method is quick, easy, and removes lipids that are known to remain on the column causing chromatographic anomalies and source contamination.


    Publication number: 5991-6771EN

    Publication Date: April 5, 2016