Online 2D-LC Characterization of Monoclonal Antibodies Using Protein A and Weak Cation Exchange Chromatography -Analysis of mAbs Using 2D-LC with High-Resolution Sampling

Version 1

    Application Note

    Biotherapeutics and Biologics

     

    Abstract

    The characterization of biopharmaceuticals can be highly complex and extensive to evaluate all important quality attributes. Online 2D-LC analysis enables a combination of different analysis methods to monitor different parameters in one run. This Application Note shows a combination of protein A titer analysis in the first dimension followed by ion exchange chromatography for charge variant profiling. The setup using high-resolution sampling provides for highly precise analysis regarding retention time and area and enables time-saving and easy monitoring for process or quality control of monoclonal antibodies.

     

    Publication number: 5991-6848EN

    Publication Date: May 1, 2016

     

    http://www.agilent.com/cs/library/applications/5991-6848EN.pdf