Multipesticides Residue Determination in Fresh Okra Using QuEchERS Sample Preparation and Gas Chromatography Tandem Mass Spectrometry

Version 1

    Application Note

    Food safety

     

    Abstract

    This application note describes the use of a quick, easy, cheap, effective, rugged, and safe (QuEChERS) AOAC sample preparation approach for the extraction and cleanup of multipesticide residues in fresh okra. The AOAC method used involves:

    • Initial extraction in a buffered aqueous/acetonitrile system

    • An extraction/partitioning step by the addition of salts

    • A cleanup step using dispersive solid-phase extraction (dispersive SPE).

     

    The pesticides to be quantified were taken from the Agricultural Product Export Promotion Council (APEDA) procedure for the export of okra through control of agrochemicals [1]. APEDA is an Indian regulatory authority for monitoring residues and export promotion activity which had described compounds to be monitored for okra export and its MRLs. Gas chromatography amenable compounds were segregated for GC-MS/MS analysis. The spiking levels for the recovery experiments were 10, 25, and 50 ng/g.

     

    Publication number: 5991-6207EN.pdf

    Publication Date: December 1, 2015

    http://www.agilent.com/cs/Library/applications/5991-6207EN.pdf