Analysis of Phencyclidine in Urine to U.S. SAMHSA Guidelines with LC/MS/MS and GC/MS

Version 1

    Application Note

    Forensics & Toxicology



    The U.S. Substance Abuse and Mental Health Services Administration (SAMHSA) guideline cutoff level for phencyclidine (PCP) in urine is 25 ng/mL. New guidelines from SAMHSA offer the option of LC/MS/MS as an alternative to GC/MS. In this study, we used Agilent Bond Elut Certify mixed-mode solid phase extraction (SPE) for sample preparation in an analysis of PCP by LC/MS/MS and GC/MS. Bond Elut Certify is ideal for PCP extraction from urine because it meets all requirements for linearity, limit of detection (LOD), accuracy, and precision. In addition, LC/MS/MS or GC/MS can be applied with the same sample preparation method, maximizing the convenience of instrument choice in the laboratory. GC/MS was used in splitless and split injection modes.


    Publication number: 5991-4575EN.pdf

    Publication Date: May 15, 2014