Integrated Transcriptomics and Metabolomics Study of Retinoblastoma Using Agilent Microarrays and LC/MS/GC/MS Platforms

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    Application Note

     

    Abstract

    This Application Note illustrates a multi-omics approach combining transcriptomics and metabolomics to study molecular events in the progression of retinoblastoma (Rb). On a set of tissue samples affected with Rb, we performed mRNA and miRNA gene expression using microarrays followed by pathway analysis to identify gene enrichment that would enable functional characterization of Rb. Transcriptomics data were collected using Agilent SurePrint G3 Human microarrays and a SureScan microarray scanner. Metabolomics data were obtained from aqueous humor, vitreous humor, and tear samples of Rb using Agilent 7200 GC/Q-TOF and Agilent 6550 iFunnel Q-TOF LC/MS systems. After feature extraction and processing using Agilent MassHunter Software, differential and multi-omics analyses were performed using Agilent GeneSpring software suite. This study demonstrates how a comprehensive understanding of a disease mechanism can be obtained using Agilent mass spectrometry (GC/MS and LC/MS) and microarray technologies along with Agilent Mass Profiler Professional and GeneSpring GX software.

     

    Publication number: 5991-6215EN.pdf

    Publication Date: , October 1, 2015

     

    http://www.agilent.com/cs/library/applications/5991-6215EN.pdf