Direct GC determination of acrylamide in water using the Agilent 7000B Triple Quadrupole GC/MS

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    Abstract

    Acrylamide (C3H5NO) is a polar, low MW molecule and its analysis by direct GC/MS is not an easy task, mainly because of low peak efficiency in chromatographic separation and no significant, low mass fragmentation in MS detection. With regard to these difficulties, direct GC/MS acrylamide analysis has never become a routine method and therefore, acrylamide is normally analyzed by GC/MS after derivatization (brominated) to limit strong peak broadening and increase the identification power of ion fragments. The aim of this study was to demonstrate the possibility of carrying out reliable direct GC analysis of acrylamide, by employing Ultra Inert technology and different chemically bonded GC columns. As reliable and sensitive MS/ MS detection was used, there was no need for derivatization. Standard solutions were prepared both in ethyl acetate and methanol so as to be easily coupled to SPE sample preparation. Studies were conducted on acrylamide standard solutions in the concentration range of 1-1000 ng/mL. A GC/MS method was developed and four different chemically bonded GC columns were tested in terms of peak shape, linearity, S/N, absolute areas, absolute RTs and carry over.

     

    Publication number: 5991-5297EN_v2.pdf

    Publication Date: October, 2014

     

    http://www.agilent.com/cs/library/applications/5991-5297EN_v2.pdf