Screening for More Than 740 Pesticide Residues in Food Using an Agilent GC/Q-TOF and an Exact Mass Pesticide Library

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    Application Note

     

    Abstract

    Six organically grown fruit and vegetable samples were extracted using the EN QuEChERS method, and these extracts were spiked with 93 pesticides at the 10 and 100 ng/mL level. The spiked extracts were analyzed using the Agilent 7200A GC/Q-TOF operating in high resolution TOF mode with electron ionization. The data were analyzed using Agilent MassHunter Qualitative Analysis Software (B.07.00) with the new Agilent Exact Mass GC/Q-TOF Pesticides Personal Compound Database and Library. Two GC methods were tried: • A 20-minute run using a 5 m column coupled to a 15 m column through a Purged Ultimate Union • A 40.5-minute method in the same configuration but with two 15 m columns In each case, the first column was backflushed at the end of the run. At the 10 ng/mL spiking level, the 20-minute and 40.5-minute methods were able to identify 97.3 % and 97.1 % of the pesticides, respectively. At the 100 ng/mL level, the two methods found 99.6 % and 99.8 % of the pesticides, respectively. The Q-TOF was able to measure molecular ion masses with better than 2 ppm accuracy when the signal/noise ratio was >10.

     

    Publication number: 5991-5633EN.pdf

    Publication Date: April 10, 2015

     

    http://www.agilent.com/cs/library/applications/5991-5633EN.pdf