PAH Analysis in Salmon with Enhanced Matrix Removal

Version 1

    Application Note

    Food Testing & Agriculture, Environmental

     

    Abstract

    Polycyclic aromatic hydrocarbons (PAHs) consist of fused benzene ring systems that resist degradation. They can be introduced to aquatic species by accumulation in the environment and cooking methods that use smoke. Analysis of PAHs in complex, high-fat food matrices can often present challenges as coextracted matrix hinders accurate quantitation in the form of interferences, matrix effects, and accumulation in the analytical flow path. Agilent Bond Elut Enhanced Matrix Removal—Lipid (EMR—Lipid) is the next generation of sample preparation products, and is used in convenient, dispersive solid phase extraction (dSPE) for highly selective matrix removal without impacting analyte recovery. This work demonstrates the effectiveness of this sample preparation methodology in the analysis of PAHs in salmon. The method delivers excellent accuracy (84 to 115%) and precision (RSD = 0.5 to 4.4%) for all 15 PAH compounds at all levels, providing a fast, robust, and effective analysis in high-fat samples.

     

    Publication number: 5991-6088EN.pdf

    Publication Date: August 21, 2015

     

    http://www.agilent.com/cs/library/applications/5991-6088EN.pdf