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mnevill
One of the metrics we use to evaluate a peptide map is sequence coverage. Sometimes we’re really only interested in a few key peptides, but other times it’s important to see the entire protein sequence. So what can we do when we don’t have 100% sequence coverage?    The first step is to review your data and the sequence of your protein to see how… (Show more)
a.fedulova
Hi Community, can your help me, I don’t find information about syringe filters 5191-5912, 5191-5913. This filters can use with aggressive organic solvents? This filters non-sterile? This filters nonpolar? What is the housing material - ultra pure polyethylene? What is the held volume? What is the sample volume? What type of connector?… (Show more)
in Sample Preparation
mnevill
Careful and consistent sample preparation is the first step to quality peptide mapping data.   Protein digestion for peptide mapping is among the most complicated sample prep to be done in when monitoring critical quality attributes – perhaps rivaled only by sample prep for glycan analysis.  Agilent’s Peptide Mapping “How to” Guide lays out a… (Show more)
olin
Dear Agilent team, do you maybe have some information on using  Enhanced Matrix Removal - Lipid kit (EMR-Lipid) for lipid separation in brain homogenate (eg. prepared in ratio 1:9 in PBS).  As brain homogenate has a lot of lipids and proteins, the Signal-to-Noise ratio of quantification of specific analytes using LC-MS is too low. Do you maybe… (Show more)
in Sample Preparation
a.fedulova
Hi Community, can you help me, what sorbent uses Agilent in Bond Elut EMR-Lipid dispersive SPE (example:5982-1010)?    Thanks.
in Sample Preparation
a.fedulova
Hello, what maximum operating pressure for Syringe filter, PTFE-HI, 13 mm, 0.45 µm (5191-5917) and Captiva Premium Syringe Filter, polypropylene housing, PTFE membrane, 15 mm diameter, 0.45 µm pore size (5190-5085)?
in Sample Preparation