• How to write a stopprogramm for an HPLC? (1260 Infinty II)

    How to write a stopprogramm for an HPLC? (1260 Infinty II) 
    last modified by ursw
  • G2455-85001 HSA Peptide Standard Mix Kit

    Hi community! I need know HSA Peptide Standard Mix Kit composition (G2455-85001). On the Agilent web site in Safety Data SheetsI don't found information about composition.   Thanks!
    last modified by a.fedulova
  • how to turn off pump if runnin out of solvent?

    Our pump don't stop if the level of solvents goes under the limit we set.   We flagged the  -prevent analys if level falls below "volume" -turn off pump if runnin out of solvent   but the system onl...
    last modified by dmarchini
  • Report to calculate RRT and rank

    Hi all, Just quickly I'm using OpenLAB CDS Acquisition version 2.3 and running SEC on a predefined method. I was hoping to be able to add ranks to the report. We assign samples that we run a grade (A / B / C / D...)...
    last modified by t0by
  • The requested Operation can not be performed with this tray.

    I am attempting to run the amino acid analysis protocol from the Woodward and Henderson paper "High-Speed Amino Acid Analysis (AAA) on 1.8 µM Reversed-Phase (RP) Columns" (https://www.agilent.com/cs/library/Supp...
    last modified by ehross3
  • How to change HPLC chromatograph properties?

    Hello everybody. I am using Agilent InfinityLab LC Series 1260 Infinity II HPLC. On top of that, it is connected to a radio-flow detector because mostly we use radiolabelled chemicals and trace them using HPLC in plan...
    last modified by chandrima
  • Calibration curve

    My calibration curve is working good and I keep checking the validity of curve by running QC samples all the time when sample are analyzed  . My question, is there minimum validity  period (expiry) of  ...
    last modified by drmirajkar
  • flexnet licensing error: -97,121

    Good Morning. Please, can you help me with a problem? When I open the off-line of the OPEN LAB CDS Chemstation Edition C.01.05 software. (35) I get the following error:   Can someone tell me what actions ca...
    last modified by edsonsamar
  • G4204-60090

    Hi community! Help me please, this G4204-81090 Low pressure mixer assembly for Agilent 1290 Infinity Quaternary Pump is a replacement G4204-60090?    I enclosing photo of the requi...
    last modified by a.fedulova
  • Collection of LC (HPLC) Resources

    Here is a "best of" collection of links to manuals and tools for HPLC on Agilent.com, for other instruments refer to Collection of Support Documents on Agilent.com.   Collection of LC (HPLC) Resources   ...
    last modified by rolanddiaz-bone
  • How to broadening the analysis Range of an Infinity II ELSD 1290 - G7102A

    I'm working with an Infinity 2  ELSD 1290 - G7102A to analyse Phospholipids in a fat sample. The range of the detector is limited to 70 000 LSU what is round about 100 µg/analyte. Is this already the p...
    last modified by chschaef1
  • Agilent approach of using ACE for Qualification

    qualification When Agilent does qualification of any Agilent HPLC/GC/GCMS using agilent software, Agilent use ACE technology for generating results. From the data integrity point of view in pharmaceutical industry, t...
    last modified by blr1981
  • Xcalibur 2.2 could not detect Agilent LC 1200

    We have Thermo Xcalibur 2.2 to control both Agilent LC1200 stack and Thermo TSQ quantum Ultra,  but Xcalibur could not recognize Agilent LC although it can see TSQ MS. it always says " pump/autosampler report a f...
    last modified by wendywen
  • RID stabilization

    I am having trouble in stabilizing my RID detector. Please provide me proper procedure for stabilization and getting good baseline in RID. Also when i am joining the column or removing the column there is no effect on...
    last modified by mojeersln
  • hplc method validation_linearity

    Goodmorning to everybody, I'm trying to validate an HPLC method for the analysis of curcuminoids in a dietary supplement preparation. I read lot of papers (and ICH guidelines) about validation but I can't understand...
    last modified by anto
  • Softgel sample preparation for HPLC

    Hi, I have to analyze curcumin and other actives in softgel of dietary supplements. I've already analyzed them using a small amount of the filler with good results. BTW my boss told me that a small amount of the analy...
    last modified by anto
  • HPLC trouble shutting down with Emower3

    HPLC-won't shutdown at end of sequence using empower 3- waters phone support says that it may not be possible with their software combined with the Agilent 1260/1290 HPLC I am using. Please let me know if anyone ...
    last modified by icohen
  • Cannabis testing lab - chemstation issues with concentrate baseline & calcs

    We have agilent 1100 series (HPLC with vacuum degasser, binary pump, autosampler, thermostated column compartment and MWD).  UV & Vis were on, along with the pump, 30 mins before the run.  Baseline was p...
    last modified by jamartellini
  • Error message (No RUN.LOG file created of current run)

    Hi there,   I am having problems with an HPLC 1100 series using ChemStation version B.03.01 SR1 on Windows XP SP3. I turn on the instrument and everything seems to be working properly. All components are green....
    last modified by erico.pereira
  • Simple example of command line instruction to run a specific method & sequence on fraction collector

    Hello all,   Does anyone have a simple example of a LC-OpenLab command line instruction to load a specific method & sequence  on a 1260 Fraction Collector?   More specifically, suppose I have just...
    Andrew Tom
    last modified by Andrew Tom