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jktalkington
Hello All, I recieved the following error during a run: Outlet Valve Position Blocked. Would this be at the pump or the purge valve? Thanks!
in Liquid Chromatography
mnevill
  Aqueous mobile phases are ripe for microbial growth.  This post discusses ways to prevent microbial growth from interfering with your chromatography.   Picking the right mobile phases can be key to developing a robust method – but good mobile phase hygiene is just as important!    Aqueous mobile phases are great breeding grounds for… (Show more)
ilopez
Hi Everyone   I had a problem with an injection valve of a 1100 Series HPLC sampler (G1313A). I exchanged the rotor seal and assembled the valve as usual, but when I performed  the Pressure Test vía Lab Advisor the valve started to leak from positions 3, 4 and from the inside. I have exchanged rotor seals many times but this is the first time a… (Show more)
in Liquid Chromatography
blr1981
qualification When Agilent does qualification of any Agilent HPLC/GC/GCMS using agilent software, Agilent use ACE technology for generating results. From the data integrity point of view in pharmaceutical industry, this is not right as the result has no linkage with raw data. Also we can never identify whether all acquired data has been processed… (Show more)
in Liquid Chromatography
aew
Hey guys, The last couple runs, the sequence stopped due to the autosampler arm's motor getting overheated (error code: Motor overtemp [EE 4027, > 70 1da9 5cd20049   0 ).   Are there any steps I can take to fix this issue?  It's a G1313A. Thanks, B
in Liquid Chromatography
hartmut
I wanted to upgrade the firmware of an Agilent 1100 bin pump, Labadvisor shows firmware's Main = A.06.01, Resident = ????. When I tried to update to A06.55, 50% of the software are downloaded, but then the system remains in "Rebooting resident". After switching the pump on and off, the old firmware is present. How can I upgrade the firmware?
in Liquid Chromatography
hafizrafique
Dear all Asslam o Alaikum ( may peace be upon all of you) I am using Agilent HPLC 1260 infinity series in regulatory analysis of pesticides. I have to induct overall uncertainty (not componental such as column oven temperature, flow rate, injection volume etc) of HPLC in my uncertainty budget of measurement. Can anybody help me out how to induct… (Show more)
in Liquid Chromatography
anto
Click to view contentHi, I have to analyze curcumin and other actives in softgel of dietary supplements. I've already analyzed them using a small amount of the filler with good results. BTW my boss told me that a small amount of the analyte in the filler could interact with the shell, so I'd like to analyze the entire softgel by solving it in the flask. As the best… (Show more)
in Liquid Chromatography
mnevill
Properly fitted connections are key to optimizing separations.  This post illustrates the effects of poorly fit connections and suggests resources to help.   Many of us don’t spend much time thinking about the fittings used to plumb our LC, including connecting the column, but this seemingly small detail can cause major headaches if they aren’t… (Show more)
anto
Hi guys, I have to analyze a sample of sodium butyrate (powder from dietary supplement) in HPLC without using phosphate buffer. I've found this method but it seems it doesn't work.. mobile phases: A water 1% formic acid; B: ACN; column T 40°C; flow 0.6 ml/min; Column Zorbax SB c18  t   %B 0   0 3   0    7   1 13   13 16   30 23   55… (Show more)
in Liquid Chromatography
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