• Agilent Seahorse Mito Fuel Flex Report Generator FAQs

    Why is the scale always 100%? Absolute OCR (pmol/min) values are transformed into % values for dependency, capacity and flexibility based on their respective definitions and mathematical operations. Thus the theoret...
    courtneynadeau
    last modified by courtneynadeau
  • Agilent Seahorse Mito Stress Test Single-File and Mulit-File XF Report Generator FAQs

    What rate measurements are used to calculate the parameters in this Report Generator? Parameter equations are described in Table 1 of the user guide.   How do I remove outlier wells in the Report Generator? Ou...
    courtneynadeau
    last modified by courtneynadeau
  • Agilent Seahorse XF Glycolytic Rate Assay Kit FAQs

    Why does the protocol call for adding buffer to the media, will it dampen the ECAR signal? A low concentration of HEPES (5 mM) has been found to provide consistent buffer capacity values across the time frame of the...
    courtneynadeau
    last modified by courtneynadeau
  • Agilent Seahorse XF Real-Time ATP Rate Assay Kit FAQs

    How is the mitoATP Production Rate calculated if there are different oxidizable substrates (with different P/O ratios) in the assay media? To convert ATP-coupled respiration (OCR) into mitochondrial ATP productio...
    courtneynadeau
    last modified by courtneynadeau
  • Agilent Seahorse XF Real-Time ATP Rate Assay Report Generator FAQs

    What rate measurements are used to calculate the parameters in this Report Generator?  Parameter equations are described in Table 1 on page 6 and Table 2 on page 17 of the Report Generator User Guide.   ...
    courtneynadeau
    last modified by courtneynadeau
  • Agilent Seahorse XF Glycolytic Rate Assay Single-File and Multi-File Report Generator FAQs

    What rate measurements are used to calculate the parameters in the Report Generator? Parameter equations are described in Table 1 on page 6 and Table 2 on page 17 of the Single File RG user guide. Parameter equatio...
    courtneynadeau
    last modified by courtneynadeau
  • Agilent Seahorse XF Cell Energy Phenotype Test Report Generator FAQs

    What rate measurements are used to calculate the parameters in this Report Generator? Parameter equations are described in Table 1 on page 6 of the user guide.   How do I remove outlier wells in the Report Gen...
    courtneynadeau
    last modified by courtneynadeau
  • Agilent Seahorse XF Mito Fuel Flex Test Kit FAQs

    What if all three inhibitors only cause a small decrease in total OCR? Processes other than oxidation of these three fuels may contribute to baseline OCR. These processes may be broken down further into mitochondria...
    courtneynadeau
    last modified by courtneynadeau
  • I have a display on my Agilent XFp Seahorse analyzer that says: Access to 'C:\ProgramData\Seahorse BIioscience.Inc\Seahorse Wave\Data\intrumentConfiguration.xml'is denied. Can anyone please advise or assist resolve this problem?

    I also can not see the Temperature reading nor can I press play to open the set-up page
    bms
    last modified by bms
  • Buffer Factor Calculator report generator?

    Hi, Where can I find the Buffer Factor Calculator report generator for the Seahorse platform? This is referenced in the Buffer Factor protocol as a file to download from the site under report generator. I can't find i...
    bjsheaha
    last modified by bjsheaha