Log in to follow, share, and participate in this community. Not a member? Join Now! We just buy a AriaMx realtime machine. We run the b-actin with SYBR 2X mixture we used before in a ABI machine. The realtime RT-PCR using the protocol 95C 2min, 95C 5seconds and 60C 30 seconds. No signal in the ... I am planning to purchase SYBR Green Master Mix for qRT-PCR. Which one is better ? Dear All I am using Mx3000p qPCR, It gives the following error "The thermal blocks or sample cover not reaching the set temperature", How I could solve it this error, thanks in advance. Teme How to design specific Forward primer for miRNA (for miRNA QRT-PCR Detection Kit)? Mentioned in the protocol: miRNA QRT-PCR Detection Kit www.genomics.agilent.com/files/LitItems/miRNA_primer_design_... Can the AriaMx be used for dsDNA quantitation? Do we have any application notes to share for the method?