INSTRUMENT CONTROL PARAMETERS: 1 ----------------------------------- Wed Feb 14 11:37:32 2024 Control Information ------- ----------- Sample Inlet : GC Injection Source : External Device Mass Spectrometer : Enabled Injection Location: Front No Sample Prep method has been assigned to this method. GC GC Summary Run Time 22.133 min Post Run Time 0 min Oven Temperature Setpoint On (Initial) 32 °C Hold Time 7 min Post Run 70 °C Program #1 Rate 10 °C/min #1 Value 70 °C #1 Hold Time 0 min #2 Rate 30 °C/min #2 Value 320 °C #2 Hold Time 3 min Equilibration Time 0.25 min Max Temperature 320 °C Maximum Temperature Override Disabled Slow Fan Disabled Front SS Inlet He Excluded from Readiness ***Excluded from Affecting GC's Readiness State*** Mode Split Heater On 250 °C Pressure On 17.915 psi Total Flow On 91.23 mL/min Septum Purge Flow On 3 mL/min Pre-Run Flow Test Off Gas Saver On 20 After 3 min mL/min Split Ratio 50 :1 Split Flow 86.5 mL/min Liner Agilent 5190-2293: 900 μL (Splitless, single taper, wool, Ultra ) Back PTV Inlet He Mode Sleep Pressure Off Septum Purge Flow Off Pre-Run Flow Test Off Liner Agilent G1544-80730: 675 μL (Direct connect, single taper, bottom ) Column Column #1 Flow Setpoint On (Initial) 1.73 mL/min Post Run 0.57353 mL/min Column Information Agilent Restek-Rxi5SilMS Temperature Range -60 °C—350 °C (350 °C) Dimensions 30 m x 250 μm x 0.25 μm Column lock Unlocked In Front SS Inlet He Out PSD 2 (Initial) 32 °C Pressure 17.915 psi Flow 1.73 mL/min Average Velocity 34.773 cm/sec Holdup Time 1.4379 min Control Mode Constant Flow Column #2 Pressure Setpoint On (Initial) 2 psi Post Run 10 psi Temperature Range -1 °C—325 °C (325 °C) Dimensions 5 m x 200 μm x 0 μm Column lock Unlocked In PSD 2 He Out MSD (Initial) 32 °C Pressure 2 psi Flow 1.5224 mL/min Average Velocity 109.14 cm/sec Holdup Time 0.076352 min Control Mode Constant Pressure Column Outlet Pressure 0 psi Front Detector FID Excluded from Readiness ***Excluded from Affecting GC's Readiness State*** Makeup He Heater On 300 °C H2 Flow Off Air Flow Off Makeup Flow Off Carrier Gas Flow Correction Constant Makeup and Fuel Flow Flame Off Detector Evaluation Perform Detector Evaluation Test Off Signal Selected Signal #1: None Checkout Sample None MSD Transfer Line Temperature Setpoint On (Initial) 320 °C Aux EPC 1,2,3 Aux EPC 1 N2 Pressure Setpoint Off (Initial) 10 psi Post Run 10 psi Excluded from Readiness ***Excluded from Affecting GC's Readiness State*** Aux EPC 2 N2 Pressure Setpoint Off (Initial) 10 psi Post Run 10 psi Excluded from Readiness ***Excluded from Affecting GC's Readiness State*** Aux EPC 3 N2 Pressure Setpoint Off (Initial) 10 psi Post Run 10 psi Excluded from Readiness ***Excluded from Affecting GC's Readiness State*** PSD 2 Excluded from Readiness ***Excluded from Affecting GC's Readiness State*** PSD Purge On 0 mL/min PSD 2 He Supplies Column 2 Signals Signal #1: Description None Signal #2: Description None Signal #3: Description None Signal #4: Description None Signal #5: Description None Signal #6: Description None Signal #7: Description None Signal #8: Description None GERSTEL MAESTRO SYSTEM SETTINGS Maestro Runtime : 22.13 min GC Cool Down Time : 5.00 min Cryo Timeout : 15.00 min GERSTEL CIS CIS : not used - use these parameters if it becomes 'used' CRYO COOLING Cryo Cooling : used TEMPERATURE PROGRAM Heater Mode : Standard Liner Type : 013882-010-00 glass liner CIS4, baffled, non deactivated Liner Max Temperature : 400 'C Initial Temperature : -10 'C Equilibration Time : 0.50 min Initial Time : 0.50 min Ramp 1 Rate : 12.00 'C/s End Temp : 325 'C Hold Time : 5.00 min Ramp 2 Rate : 0.0 'C/s Low Split : not used GERSTEL MPS PREP Sample Prep : used PREP ACTION LIST Action Method/Value Source Destination ============= =================== =================== =================== GERSTEL MPS Liquid Injection Syringe : 10ul 65mm USM SAMPLE PARAMETERS Sandwich : not used Inj. Volume : 2.0 uL Air Volume below : 0.0 uL Inj. Speed : 50.00 uL/s Fill Volume : 5.0 uL Fill Strokes : 5 Fill Speed : 2.00 uL/s Viscositiy Delay : 0 s Eject Speed : 50.00 uL/s Pre Inj. Delay : 0 s Post Inj. Delay : 0 s Inj. Penetration : 40.00 mm Sample Tray Type : VT54/2-CVM Bottom Sense : used Fast Injection : used CLEANING PARAMETERS Preclean Sample : 2 Wash Station 1 : Pos1@Standard Wash 1 Preclean Solv.1 : 2 Postclean Solv.1 : 6 Clean Volume Solv.1 : 90 % Fill Speed Solv.1 : 5.00 uL/s Viscosity Delay Solv.1 : 0 s Eject Speed Solv.1 : 50.00 uL/s Information Solv.1 : Hexane Wash Station 2 : Pos2@Standard Wash 1 Preclean Solv.2 : 0 Postclean Solv.2 : 0 Clean Volume Solv.2 : 70 % Fill Speed Solv.2 : 5.00 uL/s Viscosity Delay Solv.2 : 0 s Eject Speed Solv.2 : 50.00 uL/s MS Information -- ----------- General Information ------- ----------- Acquisition Mode : Scan Solvent Delay (minutes) : 3.3 Tune file : D:\MassHunter\GCMS\1\5977\etune.u EM Setting mode Gain : 5.000000 Normal or Fast Scanning : Normal Scanning Trace Ion Detection : Off Run Time (if MS only) : 23.67 minutes [Scan Parameters] Start Time : 3.3 Low Mass : 40 High Mass : 300 Threshold : 300 A/D Samples: : 4 [MSZones] MS Source : 230 C maximum 250 C MS Quad : 150 C maximum 200 C Timed Events ----- ------ Number Events= 1 [Timed MS Detector Table Entries] Time (min) State (MS On/off) 10.00 off END OF MS ACQUISITION PARAMETERS TUNE PARAMETERS for SN: US2018M021 --------------------------------- Trace Ion Detection is OFF. 34.593 : EMISSION 70.007 : ENERGY 3.790 : REPELLER 89.822 : IONFOCUS 20.181 : ENTRANCE_LENS 1669.658 : EMVOLTS 2061.7 : Actual EMV 5.00 : GAIN FACTOR 1872.000 : AMUGAIN 133.875 : AMUOFFSET 1.000 : FILAMENT 0.000 : DCPOLARITY 6.990 : ENTLENSOFFSET 9.502 : Ion_Body 4.006 : EXTLENS -250.000 : MASSGAIN -31.000 : MASSOFFSET END OF TUNE PARAMETERS ---------------------- END OF INSTRUMENT CONTROL PARAMETERS ------------------------------------