Size exclusion peptide with AdvanceBio SEC, 130Å, 2,7 µm, 4,6 x 300 with PL1580-5350 part number

Hi,

I passed this query on to a colleague who was involved with developing these columns, this was their response:

Column care 

An increase in backpressure and decrease in performance may occur over time. If the pressure has increased, first identify if this increase is due to a guard column that may need to be replaced. If the increase in pressure is in a system component, such as tubing or a filter, replace the component and retest. Column cleaning instructions It may be possible to restore column performance using one of the following cleaning solutions: – For strongly adsorbed contaminants: high salt concentration at low pH (for example, 0.5 M Na2 SO4 , pH 3) or 0.5 M guanidine hydrochloride – Organic solvent for hydrophobic materials: up to 50% methanol, ethanol, or isopropanol – Acidic reagents for basic contaminants: 0.1% TFA, formic acid, or acetic acid in 15% acetonitrile Always flush the column in the direction of the flow arrow, and lower the flow rate to keep the pressure below 200 bar for 2.7 µm columns or 400 bar for 1.9 µm columns. Rinse with at least five column volumes of ultrapure water before and after flushing with at least 20 column volumes of the cleaning solution.It is not recommended to use all three cleaning buffers sequentially. Choose the most appropriate buffer for your probable contaminant. Take care to avoid precipitation of buffer salts, and avoid overpressuring the column due to mobile phase viscosity differences. Recommended storage Short-term storage (less than two weeks): store the column in the mobile phase. Extended storage (longer than two weeks): store the column in filtered 100 mM sodium phosphate, pH ≤ 7, with or without 0.02% NaN3 , or 20% methanol in water. Flush the column with a minimum of 10 column volumes. To switch to or from 20% methanol, column flushing must be done at low flow rates to avoid overpressuring the column due to high viscosity. Starting at a lower flow rate, flush at no more than 0.05 mL/min for 2.1 mm id columns, 0.1 mL/min for 4.6 mm id columns, and 0.2 mL/min for 7.8 mm id columns, while also ensuring the pressure remains below 200 bar for 2.7 µm columns or 400 bar for 1.9 µm columns. Store columns at room temperature.

You may decrease flow rate to 0.35 ml/mn and increase molarity K2HPO4 150mM Ph =7.0 PH is very important  keep 7.00