I would like to measure the Vitamin B5 content in the raw material. Can anyone tell me how to measure vitamin B5 by using the HPLC-UV and which column should we choose?

Question

Currently, we find a method using HPLC from Waters so that we plan to buy a column from Waters. I don't know if this column can fit into the Agilent machine

Dr. Know · Accepted Answer

Hi Yangbei, 
 yes, those columns would fit into an Agilent LC instrument. You need to check the fitting of the column against the ones of your connection tubing on the Agilent machine, sometimes another ferrule/fitting needs to be used. There is a webinar about this here . 
 But have you had a look at our two application notes about multi-vitamin samples? 
 
 Simultaneous Detection and Quantitation of 14 Water-Soluble Vitamins in a Supplement by LC/MS/MS Triple-Quadrupole 
 Analysis of water-soluble vitamins from multivitamin tablets for nutrition labeling 
 
 Both done on Poroshell columns and very fast, Vitamin B5 eluting between 3.5 and 3.7 minutes. 
 Regards, 
 Norbert

I would like to measure the Vitamin B5 content in the raw material. Can anyone tell me how to measure vitamin B5 by using the HPLC-UV and which column should we choose?

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