Shelf life of solutions for AA analysis on HPLC

Question

Can anyone advise if the following shelf life are correct? mobile phase A (anhydrous Na2HPO4 and of Na2B4O7 water solution (1day?)) Mobile phase B (acetonitrile: methanol: HPLC grade water at a ratio of 45;45:10 v:v:v (7days?)) Injection diluent DIL, OPA, FMOC (7-10days?) borate buffer (3days) thanks

Dr. Know · Accepted Answer

Hi Manulvy, 
 there are more parameters playing a role here like white or brown glass bottles, bottles open or closed with/without StaySafe caps, temperature, etc. 
 Buffers 3 days seems ok, high organic content eluent a week is fine (still there might be evaporation). Derivatization reagents definitely not 7-10 days, 1-3 days is more likely and in the fridge when not in use with a new septum cap. 
 Just as a guideline. 
 Regards, 
 Norbert

Shelf life of solutions for AA analysis on HPLC

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